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. 2013 Aug 30;288(41):29680–29691. doi: 10.1074/jbc.M113.495069

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Vprbp silencing does not affect the proliferation of ovarian cancer cells. A, immunohistochemistry results for VPRBP expression in ovarian cancer tissues. Sections were counterstained with hematoxylin and eosin. B and C, Western blot (B) and quantitative RT-PCR (C) results for Vprbp RNAi depletion efficiency. D, Vprbp knockdown did not affect colony formation by OV2008 cells. E, Vprbp knockdown did not affect ovarian cancer cell proliferation. Cells were seeded in 96-well plates (3,000 cells/well) at 48 h after transfection and then assessed by MTT assay. F, Vprbp depletion did not induce cell cycle arrest in ovarian cancer cells. Cells were transfected with siCON or siVprbp for 72 h and then subjected to PI staining and FACS analysis to determine cell cycle profiles.