FIGURE 5.

Sost overexpression exacerbates and alendronate blunts resorption induced by PTH receptor activation in osteocytes. Intracortical porosity in the femoral diaphysis was measured by micro-CT (A) and calvarial marrow space were calculated by measuring histomorphometrically the area occupied by marrow versus bone (B). Osteoclasts were quantified in TRAP stained calvarial sections. n = 3–5 mice/group (C). Bars represent the mean ± S.D. *, p < 0.05 versus respective controls not expressing the caPTHR1 transgene and lines connect conditions with p < 0.05, by two-way ANOVA. Representative images of histologic sections showing TRAP (+) osteoclasts (D) and fluorochome labeling (E) in the calvariae of 6.0-week-old WT and DMP1–8kb-caPTHR1 mice with or without the DMP1–8kb-Sost transgene or alendronate. Bars indicate 100 μm.