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. 2013 Sep 3;288(41):29890–29900. doi: 10.1074/jbc.M113.510826

FIGURE 6.

FIGURE 6.

Knockdown of PHB2 abolishes IGFBP-6-induced Rh30 cell migration. A, Rh30 cells were treated with PHB2 or control (Con) siRNA for 72 h, followed by PHB2 and PHB1 Western blotting. Control cells without siRNA are shown for comparison. β-Actin was used as a protein loading control. The blot is representative of five independent experiments. B, migration assay was performed in the absence (Con) and presence of 1 μg/ml WT (BP6) or mIGFBP-6 (mBP6) for 24 h. Results are expressed as a percentage of the relevant control and shown as mean ± S.E. (error bars) of five independent experiments (***, p < 0.001 versus control). C, migration assay was performed as above in the absence (Con) and presence of mIGFBP-6 (mBP6; 1 μg/ml) or IGF-II (100 ng/ml) for 24 h. Results are expressed as a percentage of the relevant control and shown as mean ± S.E. of three independent experiments (*, p < 0.05; **, p < 0.01; ***, p < 0.001 versus control; ^, p < 0.05 mBP6/Phb2 siRNA versus mBP6 with no or control siRNA).