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. 2013 Sep 3;288(41):29934–29942. doi: 10.1074/jbc.M113.492520

FIGURE 6.

FIGURE 6.

The nuclear localization sequence of GAREM1. A, schematics of the full-length and deletion mutant constructs of GAREM1 and full-length GAREM2. The numbers indicate amino acid residues. B, all recombinant proteins were expressed as the N-terminal FLAG-tagged form. Each construct was transfected into COS-7 cells, and then we analyzed their localization by immunofluorescence staining using anti-FLAG antibody. Representative results are shown. The result of nuclear localization of GAREM derivatives are indicated as (+) or (−) at the right side of each schematic in A. Scale bars = 10 μm. C, amino acid sequences of the N-terminal region of GAREM1 (top) and GAREM2 (bottom). The numbers indicate amino acid residues. Lys-12 and Lys-15 in the putative nuclear localization sequence of GAREM1 (underlined) are shown in bold. D, all recombinant proteins were expressed as the N-terminal FLAG-tagged form. The results of the immunofluorescence staining using anti-FLAG antibody are indicated. The numbers indicate amino acid residues. K12/15A, double point mutant of full-length GAREM1 with Lys-12 and Lys-15 replaced with Ala.