FIGURE 2.
Bioactive MeV N-ΔC86 does not interact with a carboxyl-terminal PCT region harboring the P-X domain. Lysates of cells expressing the specified N construct and either the FLAG epitope-tagged amino-terminal PNT region (A) or full-length P or the carboxyl-terminal PCT region (B) (51) were subjected to direct immunoblotting (IB) or immunoprecipitation (IP) of PNT (α-Flag) or N (α-N). Co-precipitated (co-IP) N or P material, respectively, was detected in immunoblots using specific antibodies. Immunoblots shown are representative of two (PNT) or three (P, PCT) independent experiments. WCL, whole cell lysates.