FIGURE 5.
recMeV encoding both N-ΔC86 and standard N are replication competent. A, schematic of the genomes of standard recMeV GFP and newly generated recMeV N-ΔC86-P-N. B, immunoblot (IB) analysis of N protein expression in cells infected with recMeV N-ΔC86-P-N or standard recMeV. For comparison, total lysates of cells transfected with N-ΔC86 or standard N expression plasmids were analyzed in parallel. Control cells received infection media only (mock). C, cytopathic effect and lateral spread of cells infected with recMeV N-ΔC86-P-N or recMeV GFP, monitored by following eGFP expression in infected cells. Representative fields of view are shown at a magnification of 200×. D, stock titers of cell-associated particles of a panel of four recombinants generated for this study in comparison with standard recMeV or recMeV lacking the M protein (recMeV ΔM). Error bars represent the titer range observed.