FIGURE 6.

Inhibition of L. donovani growth and LdUPRT activity by high substrate concentrations. The abilities of wild type (■), Δcps (▴), and Δumps (●) L. donovani promastigotes to grow in various concentrations of uracil (A) or in various concentrations of uracil plus 250 μm cytidine (B), 2 mm orotate (C), or 2 mm dihydroorotate (D) were assessed as described under “Experimental Procedures.” The data are those from one of at least three independent experiments, all of which gave virtually identical results.