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Sensitivity of wild type and null mutant L. donovani to 4-thiouracil and 5-fluorouracil. The abilities of wild type (■), Δuprt (♦), Δcps (▴), and Δumps (●) L. donovani promastigotes to grow in various concentrations of 4-thiouracil (A) or 5-fluorouracil (B) were assessed as described under “Experimental Procedures.” Growth data are presented from a single representative experiment, which has been repeated at least three times with similar results.
