FIGURE 7.

Levels of pro- and anti-inflammatory cytokines in macrophages infected with HbN-expressing MtbRa. A, levels of IL-6 secretion in Mtb-infected mouse peritoneal macrophages. 15- and 30-day grown cells of wild type and HbN-overexpressing cells of MtbRa were infected in mouse peritoneal macrophages at MOIs (shown in the inset) of 1:5, 1:10, and 1:20. Levels of IL-6 were measured in culture supernatant after 48 h. The data are represented as bars in the following order: uninfected, wild type MtbRa-Wt (15 days), MtbRa-HbN (15 days), MtbRa-Wt (30 days), and MtbRa-HbN (30 days). B, levels of TNF-α in Mtb-infected mouse peritoneal macrophages. 15- and 30-day grown cells of wild type and HbN-expressing cells of MtbRa were infected into mouse peritoneal macrophages at an MOI of 1:20, and the level of TNF-α in Mtb-infected macrophages was measured in culture supernatant. The data are represented as bars in the following order: uninfected, wild type MtbRa-Wt (15 days), MtbRa-HbN (15 days), MtbRa-Wt (30 days), and MtbRa-HbN (30 days). Results shown as mean ± S.D. (error bars) are representative of three independent experiments and are expressed as pg/ml.