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. 2013 Oct 11;7:179. doi: 10.3389/fncel.2013.00179

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Copyright © Bouzier-Sore and Pellerin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Typical high resolution at the magic angle spinning (HRMAS) ¹H-NMR spectrum of rat brain biopsy after [3-¹³C]lactate perfusion. Protons of the methyl group of lactate are detected (black arrows), centered at 1.32 ppm. The doublet is coming from the homonuclear spin coupling (J_H-H = 7 Hz, red arrows). When a ¹³C is located on lactate carbon 3, then a doublet of doublet is appearing (¹³C satellites of H₃ lactate), due to the heteronuclear spin coupling (J_H-C = 128 Hz, horizontal blue arrows).