FIGURE 2.
Typical high resolution at the magic angle spinning (HRMAS) 1H-NMR spectrum of rat brain biopsy after [3-13C]lactate perfusion. Protons of the methyl group of lactate are detected (black arrows), centered at 1.32 ppm. The doublet is coming from the homonuclear spin coupling (JH-H = 7 Hz, red arrows). When a 13C is located on lactate carbon 3, then a doublet of doublet is appearing (13C satellites of H3 lactate), due to the heteronuclear spin coupling (JH-C = 128 Hz, horizontal blue arrows).