Fig. 3.

The size of the intracellular Bem3 compartment is proportional to the expression levels of Bem3. Cells expressing either HA-Bem3 or GFP-Bem3 from a GAL1 promoter were grown overnight at 30°C in medium containing 2% glucose, transferred to 2% galactose-containing mediim and aliquots obtained for western blotting (anti-HA antibody) or microscopy (100×, FITC filter) at the indicated times. Scale bar: 5 µm. The size of the Bem3-containing compartment was measured using ImageJ as described before. Horizontal lines on the graph indicate median values of the size of the Bem3-containing compartment for the respective induction times.