Fig. 6.
The area of the Bem3-containing compartment is enlarged in cells experiencing extended periods of apical growth. (A) Diploid cells expressing GFP-Bem3 were grown in the absence (Control) or presence (Pseudohypha) of 1% butanol for 6 hours and imaged at 100× using a FITC filter. (B) cdc3ts mutant cells transformed with Yep13-GFP-Bem3 were grown at 24°C or shifted to 37°C for 6 hours before imaging at 100× using a FITC filter. The areas of the Bem3-containing compartments of control and pseudohypha-induced cells were quantified using ImageJ. Statistical significance was tested using the Wilcoxon test (**P<0.001). Scale bar: 5 µm.