Table 1. Colocalization of the Bem3-containing compartment with intracellular markers.

Images were obtained using GFP (Bem3) and Rhodamine (marker) filters. If the intensity value in the red channel was above the bleedthrough/crosstalk thresholds, a colocalization value of 1 was assigned to that particular structure; otherwise a score of 0 was assigned. At least 50 cells were analyzed per marker. Percentage colocalization was obtained as (sum of colocalization values/total number of compartments analyzed)×100.