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. 2013 Oct 15;126(20):4647–4658. doi: 10.1242/jcs.126573

Fig. 7.

Fig. 7.

Rab40b is required for MDA-MB-231 cell invasion in vitro. (A,B) Mock- or siRNA-treated MDA-MB-231 cells were plated on matrigel-coated (A) or uncoated (B) 8-µm-pore filters. Cells were then incubated for 8 hours (B) or 16 hours (A) and the extent of cell migration to the bottom side of the filter was analyzed by Crystal Violet staining (see the Materials and Methods). Data shown are the means and s.d. of three independent experiments; *P>0.05. (C–H) MDA-MB-231 cells were plated on gelatin and fibronectin-HiLyte Fluor488-coated coverslips (D,E,G and H). After incubation for 20 hours, cells were fixed and stained with Rhodamine-phalloidin (C,E,F,H). Arrows indicate invadopodia. Scale bars: 5 µm (C–E), 1 µm (F–H). (I–K) MDA-MB-231 cells were plated on gelatin-coated coverslips. After incubation for 20 hours, cells were fixed and stained with Rhodamine-phalloidin (I,K) and rabbit anti-Tks5 antibodies (J,K). Scale bar: 1 µm.