FIGURE 1.

Selection of an endogenous control for the analysis of MV-infected human PBMCs. (A) RNA was extracted from spleen cells of hNOJ and non-humanized NOJ, and one-step qRT-PCR was performed using primer and probe sets designed against the human-specific hCD45 and RNaseP mRNAs. To calculate copy numbers of these genes, the PCR products of human CD45 and RNase P were subcloned into plasmids and used as standard DNAs. (B) Human PBMCs from five donors were fractionated into CD14⁺ monocytes and T cells. RNA from these cell populations was extracted, and the expression levels of hCD45 and RNase P were analyzed by qRT-PCR. The graph depicts the expression levels in these fractionated cells relative to the levels in PBMCs (defined as 1). Statistical differences in hCD45 and RNase P expression among these cell populations were evaluated by non-parametric one-way ANOVA test (*P<0.05).