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. 2013 Jul 24;5(3):901–918. doi: 10.3390/cancers5030901

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Analysis of pro- and anti-apoptotic proteins in response to PHA-767491. KMS-18 and MM1S myeloma cells were incubated with 5 μM PHA-767491 for the indicated time. Protein extracts were prepared and analyzed by immunoblotting using the indicated antibodies (A). In parallel samples, apoptosis (B) and DNA synthesis (C) were analysed by flow cytometry using AV staining and EdU incorporation assay respectively. Numbers in the gated regions represent the percentage of cells positive for either AV (B) or EdU (C) staining.