Fig. 2.

mRNA expression of TNF and IDO production of AMs and PMs in response to LPS. (A) GeNorm (qBaseplus software) was used for the ranking of the six candidate reference genes based on their M value. All reference genes had values less than 1.5 which is the cutoff point under which genes are considered to be stably expressed. 18S was identified as the most stable gene. Quantitative PCR of the TNF (B) and IDO (C) transcripts, respectively, were performed on cDNA samples of both AMs and PMs and mRNA expression was normalised to 18S. Results are the mean of a minimum of 4 experiments ± SEM (*p < 0.05 versus control 0 h).