Fig. 4.
Effects on NO/cGMP signaling. (A) 10,000 g supernatants of hearts from WT (open bars), ATGL(−/−) (solid bars), WT/MHC-A35 (striped bars), and ATGL(−/−)/MHC-A35 mice (gray bars) were prepared and assayed for Ca2 +-dependent [3H]l-citrulline formation. Data are mean values ± S.E.M. of 4 experiments performed in duplicate. (B) Cardiac sGC activity was measured in cytosols stimulated with 0.1 mM DEA/NO with and without YC-1 (0.1 mM). The inset depicts basal enzyme activity assayed in the presence of MnCl2. Data are mean values ± S.E.M. of 5 experiments performed in duplicate. *p < 0.05 ATGL(−/−) vs WT. (C) cGMP levels were determined by radioimmunoassay. Data represent mean values ± S.E.M. of 5–7 experiments performed in duplicate. (D) Phosphorylation of VASP at serine 239 was quantified by Western blot. Data are presented as the ratio of phosphorylated to total VASP protein and represent mean values ± S.E.M. of 6 individual experiments.