Fig. 6.

Systemic application of MnTBAP (5 mg × kg^− 1 × day^− 1) aggravates oxidative stress in ATGL deficiency. (A) gp91ds-tat-sensitive lucigenin chemiluminescence was measured in cardiac homogenates prepared from MnTBAP-treated WT (open bars) and ATGL(−/−) mice (solid bars) and compared to that of non-treated animals. Data represent mean values ± S.E.M. of 4 individual experiments. *p < 0.05 non-treated and MnTBAP-treated ATGL(−/−) vs non-treated WT; §p < 0.05 non-treated ATGL(−/−) vs MnTBAP-treated ATGL(−/−). (B, C) Cardiac BH₄ and BH₂ were determined by HPLC. Data represent mean values ± S.E.M. of 4 individual experiments. *p < 0.05 ATGL(−/−) vs WT.