Skip to main content
. 2012 Nov 26;32(41):4892–4902. doi: 10.1038/onc.2012.517

Figure 3.

Figure 3

Notch-mediated IL-6 upregulation is dependent on the cellular p53 status. (a) IL-6 mRNA expression was not significantly upregulated in MCF7 cells following infection with adenoviral vectors expressing Notch1 ICD (N1ICD) or enhanced green fluorescent protein (EGFP) (control). (b) Western blot analysis of p53 protein levels in MDA-MB-231 cells after transfection of empty pCMX vector (control) or wild-type p53 (wt p53). (c, d) IL-6 (c) and Hes1 (d) mRNA expression was analyzed following infection with adenoviral vectors expressing EGFP (control), Notch1 ICD or Notch3 ICD in MDA-MB-231 cells, combined with transfection of wt p53 or empty pCMX vector as control, as indicated. (e) p21 mRNA expression or p53-luciferase activation in MDA-MB-231 cells transfected with wt p53 or control plasmid (pCMX). (f) IL-6 mRNA expression analyzed in p53−/− mouse embryonic fibroblasts (MEFs) transfected with Notch1 ICD or EGFP plasmid and co-transfected with either wt p53 or empty pCMX vector, as indicated. (g) Analysis of IL-6 mRNA expression in MCF7 cells transfected with pCMX, Notch1 ICD or p53R248W (mut p53) co-transfected with Notch1 ICD plasmid, as indicated. (h) Comparison of IL-6 mRNA expression levels in breast tumors stratified according to p53 status (p53 wild-type versus p53 mutated/deficient, right bar) or ER status (ER+ or ER–, left bar). The median is shown as a horizontal line. Values are significant at ***P<0.001, **P<0.01 and *P<0.05, as indicated in the figure. Graphs represent averages of three independent experiments. n.s., not significant. RLU, relative luciferase units.