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. 2012 Nov 26;32(41):4892–4902. doi: 10.1038/onc.2012.517

Figure 4.

Figure 4

Notch regulates distinct gene sets in MDA-MB-231 and MCF7 cells. (a) A heat map (with triplicates) showing regulation of genes in MDA-MB-231 or MCF7 cells cultured on immobilized Jagged1 or Dll4 ligand or Fc fragment, as indicated. The γ-secretase inhibitor DAPT was added to block Notch signaling as indicated. Red=increased gene expression; green=reduced gene expression. (b) Principal component analysis of the data presented in a, as indicated. (c–e) Quantitative PCR analysis of genes that were (c) upregulated in MCF7 but not in MDA-MB-231 cells; (d) upregulated in MDA-MB-231 but not MCF7 cells; and (e) the genes from d also following transfection of wild-type p53. Values are significant at ***P<0.001, **P<0.01 and *P<0.05, as indicated in the figure. Graphs represent averages of three independent experiments.