Figure 3.

Stern-Volmer plots for the IgG1 and IgG2 mAbs as a function of excitation wavelengths at different temperatures. The line connecting the data points are not mathematical fits but simply connect the mean of the experimental data points and are used for visual aid purposes only. Samples were at 0.1 mg/mL in 20 mM citrate-phosphate buffer, pH 5.0. Each data point represents mean and standard deviation of three (n = 3) replicates. [Color figure can be viewed in the online issue, which is available at http://wileyonlinelibrary.com.]