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Effect of temperature on the acrylamide quenching profiles for the IgG1 and IgG2 plotted at 292 nm (A and C) and 304 nm (B and D) excitation wavelengths. The lines are drawn for visual aid purposes only. Samples were at 0.1 mg/mL in 20 mM citrate-phosphate buffer, pH 5.0. Each data point represents mean and standard deviation of three (n = 3) replicates. [Color figure can be viewed in the online issue, which is available at http://wileyonlinelibrary.com.]
