Table I.

Structural Transitions Observed by Various Biophysical Techniques Employed in This Study

Sr. no.	Technique	Type(s) of structural transitions monitored
1	Fluorescence spectroscopy	Tertiary structure stability by monitoring Trp peak position shifts as a function of an experimental variable(s)
2	Red-edge excitation (REE)	Distinct solvent-exposed regions by monitoring Trp peak position shifts as a function of excitation wavelength and an experimental variable(s)
3	ANS binding	Exposure of apolar regions/patches in proteins by measuring alterations in ANS fluorescence
4	Circular dichroism spectroscopy	Alterations in secondary structures by monitoring molar ellipticity
5	Differential scanning calorimetry	Overall conformational (thermal) stability by monitoring heat absorbed or released during transitions/structural alterations
6	Static light scattering	Aggregation propensity (time-averaged measurement) by monitoring changes light scattering intensity
7	Stern-Volmer analysis	Structural alterations in local environments of fluorophore; qualitative and quantitative analysis of slopes of plots constructed by plotting extent of quenching versus quencher concentration