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. 2013 Aug 1;22(10):1392–1398. doi: 10.1002/pro.2325

Figure 2.

Figure 2

Functional characterization of hTEE-658. (A) Luciferase production driven by hTEE-658 compared to the average of 9 in vitro selected human TEEs and four randomly chosen human sequences from a naïve library. Results from the naïve library are equivalent to the SLP promoter alone. (B) Luciferase mRNA levels determined by qRT-PCR after normalization to HPRT. (C) Luciferase activity normalized to cellular mRNA. (D) Reporter constructs containing 5′ and 3′ deletions were used to identify the core functional domain of hTEE-658. Labels indicate the precise nucleotide fragment analyzed in vaccinia-infected cells. Relative enhancement is given as a percentage of full-length hTEE-658 with normalized percent error shown in parenthesis. (E) Luciferase mRNA and protein levels observed for vectors carrying and lacking the vaccinia SLP promoter upstream of hTEE-658. (F) 5′ RACE analysis was used to identify the viral promoter region (underlined) and ribosomal TEE (boxed) within the core functional region of hTEE-658.