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. Author manuscript; available in PMC: 2013 Oct 11.
Published in final edited form as: Reproduction. 2010 Jul 28;140(4):605–612. doi: 10.1530/REP-10-0263

Figure 4. Effects of E2, MPA, TNF-α and IL-1β on IL-11 mRNA levels in decidual cell monolayers.

Figure 4

Confluent leukocyte-free first trimester decidual cells were incubated for 7 days in 10−8 M E2 or 10−8 M E2 + 10−7 M MPA, then switched to DM with corresponding steroid(s) ± 1 ng/ml of TNF-α or IL-1β for 6 hours. Aliquots of extracted total RNA were used to measure IL-11 mRNA levels by quantitative real-time RT-PCR. Bars represent mean ± SEM of IL-11 mRNA/β-actin mRNA levels (n=5 separate patients’ decidual specimens). * versus E2 or E2 + MPA; ** vs. E2 + IL-1β; p<0.05.