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. Author manuscript; available in PMC: 2013 Oct 11.
Published in final edited form as: Virology. 2009 Jan 8;385(1):275–281. doi: 10.1016/j.virol.2008.12.009

Fig. 5.

Fig. 5

Reactivity of anti-gp120 and anti-gp41 MAbs with cleaved and uncleaved gp140 trimers. Gel filtration-purified cleaved (SOSIP.R6 G-MPER) or uncleaved (SOSIP.IEGR GMPER) gp140 trimers were immunoprecipitated with various MAbs, and then analyzed by SDS-PAGE and western blotting. (A) Anti-gp120 MAbs and the CD4-IgG2 protein were tested. (B) MAb 17b to a CD4-induced epitope was tested in the presence or absence of sCD4. (C) Anti-gp41 MAbs were tested. (D) Several of the anti-gp41 MAbs were re-tested for binding to cleaved gp140 trimers, but now in the presence of SDS.