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. 2013 Oct 11;8(10):e77386. doi: 10.1371/journal.pone.0077386

Figure 2. Fusion protein toxicity assays in T-84 cells using cAMP and cGMP EIA ELISA kits.

Figure 2

200 ng or 2 µg of the refolded toxoid fusion protein 3xSTaA14Q-tmLT were incubated with T-84 cells, and intracellular cAMP (panel A) and cGMP (panel B) levels (pmol/ml) were measured. T-84 cells incubated with the toxoid fusion stimulated significant less cAMP (p<0.01) or cGMP (p<0.01) compared to those incubated with 2 ng STa or 10 ng CT.