Figure 1. Characterizations of TSP LEL.

A. BmTSP LEL is expressed on the cuticle of B. malayi L3. Presence of BmTSP LEL on the surface of B. malayi L3 was demonstrated by staining L3 with mouse anti-BmTSP LEL antibodies followed by FITC conjugated anti-mouse IgG (1,2). Green fluorescence denotes regions where antibody was bound. No fluorescence found when L3 was stained with control negative sera (3). Scale bar is 11µm. B. Multiple sequence alignment of Tetraspanin was performed using clustalW online tools. Sequence alignment showed that LEL domain of WbTSP is 100% similar to BmTSP and 97% similar to OvTSP. Arrow showing only two amino acids (C₃₅-Y₃₅ /D₄₈ -G₄₈) of OvTSP LEL was different from BmTSP LEL and/or WbTSP LEL. C. Immunoblot was performed by probing anti-His tag, anti-BmTSPLEL or anti-WbTSPLEL antibodies. Both anti-WbTSPLEL and anti-BmTSP LEL antibodies cross reacted with rOvTSP LEL proteins.