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. 2013 Oct 11;8(10):e77394. doi: 10.1371/journal.pone.0077394

Figure 4. TSP LEL specific IgG antibodies in the sera of mice.

Figure 4

Mice were immunized four times at two weeks interval using 15 µg of rWbTSP LEL combined with alum adjuvant. A) Titer of anti-TSP LEL IgG antibodies. Approximately, 100 ng of recombinant proteins (100 ng /100µl /well) were coated onto the wells of an ELISA plate and bound serum IgG was detected using an HRP-labeled anti-mouse IgG secondary antibody. Each data point indicates mean ±S.D value from five animals. B). Isotype of anti-TSP LEL IgG antibodies in the sera of mice. Isotypes specific ELISA was performed as described in the methods section. Bars represent mean ±SD from five mice per group. ** Significant (P<0.001) and * (P<0.05) compared to control groups (Student’s t-test).