Table 2. Primers used to identify and sequence viruses isolated from mosquitoes in this study.
| Primers | Primers sequence (5′ to 3′) | Size, base pairs |
|---|---|---|
| Alphavirus | ||
| M2W | YAGAGCDTTTTCGCAYSTRGCHW | |
| cM3W | ACATRAANKGNGTNGTRTCRAANCCDAYCC | 434 |
| M2W2 | TGYCCNVTGMDNWSYVCNGARGAYCC | |
| GETVCaF | TGGTCCCGCATCGGATTGAG | |
| GETVCaR | CTTTCATGTCATGCCCGGCGATG | 1450 |
| GETVE2F | GTAACAATAGTGCACGCCACC | |
| GETVE2R | GGCAGCAGCAAAGCAGGTTC | 1400 |
| Flavivirus | ||
| FU1 | TACCACATGATGGGAAAGAGAGAGAA | |
| cFD2 | GTGTCCCAGCCGGCGGTGTCATCAGC | 310 |
| JEPrM251F | CGTTCTTCAAGTTTACAGCATTAGC | |
| JEPrM743R | CGYTTGGAATGYCTRGTCCG | 492 |
| JEPrM925R | CCYRTGTTYCTGCCAAGCATCCAMCC | |
| JE955F | TGYTGGTCGCTCCGGCTTA | |
| JE2536R | AAGATGCCACTTCCACAYCTC | 1581 |
| Bunyavirus | ||
| BUP | ATGACTGAGTTGGAGTTTGATGTCGC | |
| BDW | TGTTCCTGTTGCCAGGAAAAT | 250 |
| Densovirus | ||
| DNV-3F | TGTCTCTTTCTCTTGGTATTTCTTC | |
| DNV-3R | CATACTACACATTCGTCCTCCAC | 903 |
| Yunnan obivirus | ||
| YUOVSeg7S1 | AGCATTCGGTACGCAGTATCTCG | |
| YUOVSeg7R1 | GCCGAGCCGATCATGTCACG | 453 |
| YUOVSeg7S2 | GAACGACAACGCATTTTGA | |
| YUOVSeg7R2 | CACGTTGTTCCTGCACTTGGTCTG | 369 |
| Orbivirus | ||
| 6-1-2F/230F | ATGCGTATGGAGGAAGAG | |
| 6-1-2R/1202R | AAAGTTGAGGCACCAGTA | 972 |
| Oya virus | ||
| Oya-S-21f | GACGCCGAGGCCCAATGTTA | |
| Oya-S-334r | CAAGCGGGAAGAAGGTGAATGTC | 313 |
Note: F means forward primer; R means reverse primer. M: C/A; W: A/T; Y: C/T; K: G/T; R: G/A; V: G/A/C; D: T/