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. 2013 Oct 11;8(10):e77380. doi: 10.1371/journal.pone.0077380

Table 2. PCR primers used in this study.

Primer Sequence (5’3’)
GPH1BamHIf CGCGGATCCTGAACAATGCCGCCAGCTAGTAC-3´
GPH1ClaIr CCCATCGATAGTCACTGGTTCAACGTTCCAAATG-3´
RSB1BamHIf CGCGGATCCGGTGGTATGGTACCGAACCTTC-3´
RSB1SalIr CCCGTCGACAAGTTTAGCCTTCTTTTTAGAGGAAAC-3´
GPM2BamHIf CGCGGATCCATGACTGCAAGCACACCATCCAA-3´
GPM2HindIIIr CCCAAGCTTAGGATTTTTTATGAAACCCTCATTACGG-3´
Act1-RTfwd CCAGCCTTCTACGTTTCCATCCAAG
Act1-RTrev GACGTGAGTAACACCATCACCGGA
Psd2_RTfw GCGCCACAAGATTATCACCGGTTTCACTC
Psd2_RTrev CTAACTCACTACGAACGGCCATTGGATTTACAG
Ale1_RTfw CTTGCGAGAAGATGGTGTCACTCCTTTG
Ale1_RTrev GGTTGCACCATGTAACCAAATGCTAGTTTAATTG
Eki_RTfw CAATCTAATCATCGAATGGAGGCCTTGTACAC
Eki_RTrev CATCACCTAGTCCAGCCTTTTCGCTC
Ect1_RTfw GCCGTTATTATCGATGCTGACGCCAC
Ect1_RTrev GTCAAGTATTCACTGAATTTTCCGGCAGCAG
Ept_RTfw CTCTTTCATCCTTTCCGTTGGTTTCACGGGAG
Ept_RTrev CATGGGTGCATTGAACATGGGAAAGCTC
Dpl1_RTfw GTCGTGCCAAGAAATAGTCGGTGCAGCAATG
Dpl1_RTrev GACTGAATATCTAGGGTTGCCCATTATATTCAGGTCTG
Gph1_RTfw CCACGCAAGGTTTCAATCTTTGGTGGTAAGAG
Gph1_RTrev CGTCGTTATTAACAATGTCAGCAACACAGTTGATC
Gpm2_RTfw GTGGGCCATGGAAGTTCAGTGAGATC
Gpm2_RTrev CAACGACTAAGGGGATACCATTTGGAATATCAAC
Rsb1_RTfw GATGGCCATCGCTACGGTCACTTTG
Rsb1_RTrev GTAAAGTTTCAGGATCAACATGGCCCGGTG

Upper part: PCR primers used for the amplification of genes. Recognition sites for restriction enzymes are underlined. Lower part: Primers used for qRT-PCR.