Table 2. PCR primers used in this study.
Primer | Sequence (5’3’) |
---|---|
GPH1BamHIf | CGCGGATCCTGAACAATGCCGCCAGCTAGTAC-3´ |
GPH1ClaIr | CCCATCGATAGTCACTGGTTCAACGTTCCAAATG-3´ |
RSB1BamHIf | CGCGGATCCGGTGGTATGGTACCGAACCTTC-3´ |
RSB1SalIr | CCCGTCGACAAGTTTAGCCTTCTTTTTAGAGGAAAC-3´ |
GPM2BamHIf | CGCGGATCCATGACTGCAAGCACACCATCCAA-3´ |
GPM2HindIIIr | CCCAAGCTTAGGATTTTTTATGAAACCCTCATTACGG-3´ |
Act1-RTfwd | CCAGCCTTCTACGTTTCCATCCAAG |
Act1-RTrev | GACGTGAGTAACACCATCACCGGA |
Psd2_RTfw | GCGCCACAAGATTATCACCGGTTTCACTC |
Psd2_RTrev | CTAACTCACTACGAACGGCCATTGGATTTACAG |
Ale1_RTfw | CTTGCGAGAAGATGGTGTCACTCCTTTG |
Ale1_RTrev | GGTTGCACCATGTAACCAAATGCTAGTTTAATTG |
Eki_RTfw | CAATCTAATCATCGAATGGAGGCCTTGTACAC |
Eki_RTrev | CATCACCTAGTCCAGCCTTTTCGCTC |
Ect1_RTfw | GCCGTTATTATCGATGCTGACGCCAC |
Ect1_RTrev | GTCAAGTATTCACTGAATTTTCCGGCAGCAG |
Ept_RTfw | CTCTTTCATCCTTTCCGTTGGTTTCACGGGAG |
Ept_RTrev | CATGGGTGCATTGAACATGGGAAAGCTC |
Dpl1_RTfw | GTCGTGCCAAGAAATAGTCGGTGCAGCAATG |
Dpl1_RTrev | GACTGAATATCTAGGGTTGCCCATTATATTCAGGTCTG |
Gph1_RTfw | CCACGCAAGGTTTCAATCTTTGGTGGTAAGAG |
Gph1_RTrev | CGTCGTTATTAACAATGTCAGCAACACAGTTGATC |
Gpm2_RTfw | GTGGGCCATGGAAGTTCAGTGAGATC |
Gpm2_RTrev | CAACGACTAAGGGGATACCATTTGGAATATCAAC |
Rsb1_RTfw | GATGGCCATCGCTACGGTCACTTTG |
Rsb1_RTrev | GTAAAGTTTCAGGATCAACATGGCCCGGTG |
Upper part: PCR primers used for the amplification of genes. Recognition sites for restriction enzymes are underlined. Lower part: Primers used for qRT-PCR.