Table 6. Phospholipid composition of cell-free homogenate, mitochondria and plasma membrane from cells grown on YPD.
| Phospholipids in cell-free homogenate, mitochondria and plasma membrane (mol %) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| CF | Strain | LPL | PI | PS | PC | PE | CL | DMPE | PA |
| Homogenate | WT | 1.52 ± 0.21 | 9.93 ± 3.53 | 8.75 ± 0.51 | 45.08 ± 1.82 | 26.61 ± 2.53 | 3.35 ± 0.29 | 4.43 ± 0.70 | 0.71 ± 0.38 |
| Δpsd1 | 2.15 ± 0.76 | 16.00 ± 1.17 | 11.28 ± 2.38 | 46.68 ± 2.34 | 18.23 ± 1.23 | 1.50 ± 0.64 | 2.75 ± 0.44 | 0.95 ± 0.23 | |
| Δgpm2 | 1.03 ± 0.64 | 13.07 ± 5.34 | 7.73 ± 1.83 | 46.63 ± 3.92 | 23.40 ± 2.46 | 3.94 ± 0.84 | 2.75 ± 1.19 | 0.90 ± 0.50 | |
| Δgph1 | 0.62 ± 0.33 | 14.04 ± 0.74 | 9.18 ± 0.85 | 39.30 ± 1.01 | 25.66 ± 0.47 | 2.13 ± 0.37 | 6.85 ± 0.52 | 1.73 ± 1.30 | |
| Δrsb1 | 1.04 ± 0.13 | 17.16 ± 1.14 | 10.50 ± 1.03 | 40.36 ± 0.08 | 22.64 ± 0.91 | 2.84 ± 0.50 | 3.51 ± 0.62 | 1.76 ± 0.62 | |
| Mitochondria | WT | 1.92 ± 1.11 | 8.06 ± 1.72 | 4.07 ± 0.60 | 40.65 ± 2.38 | 30.35 ± 1.36 | 4.97 ± 3.64 | 6.58 ± 2.54 | 2.38 ± 0.60 |
| Δpsd1 | 2.12 ± 0.46 | 8.80 ± 3.67 | 7.31 ± 1.54 | 46.98 ± 5.42 | 23.86 ± 3.96 | 2.87 ± 1.77 | 3.23 ± 1.56 | 4.78 ± 2.21 | |
| Δgpm2 | 1.53 ± 0.44 | 10.79 ± 2.45 | 3.68 ± 1.02 | 45.14 ± 2.27 | 26.98 ± 1.13 | 7.34 ± 1.79 | 1.56 ± 0.35 | 2.73 ± 0.62 | |
| Δgph1 | 1.58 ± 0.68 | 9.49 ± 2.30 | 6.94 ± 1.00 | 35.90 ± 2.32 | 33.94 ± 0.38 | 6.17 ± 1.51 | 3.66 ± 0.26 | 2.10 ± 0.58 | |
| Δrsb1 | 1.12 ± 0.11 | 13.33 ± 0.52 | 5.29 ± 0.44 | 34.86 ± 1.24 | 29.15 ± 1.13 | 8.18 ± 0.26 | 5.70 ± 0.25 | 2.05 ± 0.20 | |
| Plasma membrane | WT | 2.26 ± 0.96 | 12.38 ± 2.12 | 26.18 ± 2.79 | 18.18 ± 1.38 | 32.06 ± 4.14 | 0.69 ± 0.10 | 2.23 ± 0.43 | 5.76 ± 0.61 |
| Δpsd1 | 2.80 ± 0.53 | 17.11 ± 4.11 | 27.27 ± 3.20 | 21.27 ± 3.32 | 23.47 ± 3.61 | 0.64 ± 0.32 | 1.90 ± 0.30 | 5.30 ± 2.18 | |
| Δgpm2 | 2.19 ± 0.70 | 12.90 ± 1.71 | 24.31 ± 3.02 | 21.36 ± 1.93 | 30.94 ± 3.25 | 0.73 ± 0.34 | 1.91 ± 0.64 | 5.42 ± 1.26 | |
| Δgph1 | 1.88 ± 0.96 | 14.82 ± 2.93 | 24.90 ± 3.96 | 11.04 ± 2.25 | 36.27 ± 2.77 | 0.58 ± 0.27 | 2.44 ± 0.55 | 7.84 ± 3.13 | |
| Δrsb1 | 1.49 ± 0.40 | 13.63 ± 0.44 | 24.74 ± 2.98 | 20.17 ± 1.44 | 31.47 ± 2.71 | 0.12 ± 0.17 | 2.21 ± 0.62 | 5.72 ± 0.60 | |
CF, cellular fraction; LPL, lysophospholipids; PI, phosphatidylinositol; PS, phosphatidylserine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; CL, cardiolipin; DMPE, dimethylphosphatidylethanolamine; PA, phosphatidic acid. Mean values of at least three measurements and standard deviations are shown.