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. 2013 Oct 11;8(10):e77754. doi: 10.1371/journal.pone.0077754

Figure 3. Experimental EHV 5 infection; polarized light microscopy, picrosirius red histochemistry (horse C1, E1).

Figure 3

The collagen-specific dye, picrosirius red binds to collagen and fluoresces under polarized light. Within the lung parenchyma, collagen within control horse lung (C1) is restricted to branch points of alveoli and surrounding airways and blood vessels (A). Collagen is significantly increased in the alveolar parenchyma of the lungs of EHV 5 infected horses (horse E1, B). Magnification: A, B - 10x.