Table 3. Tissues were positive for EHV-5 using PCR assays followed by nucleic acid sequencing.
| Tissue type | C1 | C2a | E1* | E2 | E3 | E4** | E5 | E6 |
|---|---|---|---|---|---|---|---|---|
| Bronchiolar lymph node | − | VC | V | − | − | B | − | − |
| Submandibular lymph node | − | VC | V | − | − | − | − | − |
| Mesenteric lymph node | − | − | − | − | − | − | − | V |
| Lung | − | − | − | − | V | − | − | − |
| Nasal mucosa | − | − | V | − | V | − | − | − |
| Kidney | − | VC | − | − | − | − | V | − |
| Spleen | − | − | − | V | − | − | − | − |
The time from infection to post mortem examination were 14 days for C1, 97 days for C2, 98 days for E1 and E2, 105 days for E4 and E6, and 108 days for E3 and E5.
a Horse C2 was naturally infected with an EHV-5 that was designated as VC. This horse was in contact with other horses in the study before separate housing was initiated.
* Horses E1, E2, and E3 were experimentally infected with an EHV-5 designated as A
** Horses E4, E5, and E6 were experimentally infected with an EHV-5 designated as B -; negative; V: several different EHV-5 were detected in blood from the horses, those viruses are collectively designated as V