Figure 6. IL-8 release by HEK293T cells expressing murine TLR4, CD14, and MD2.

Cells were stimulated with serum, right lung BAL fluid, or left lung BAL fluid collected from mice subjected to 1 hr of left lung ischemia followed by 4 hr of reperfusion. Cells were treated with medium only for a negative control or with LPS (10 ng/ml) for a positive control. Each experiment was carried out with and without polymixin B (50 µg/ml).