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. 2013 Oct 11;8(10):e76767. doi: 10.1371/journal.pone.0076767

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© 2013 Burtnick, Brett

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Whole cell lysates were prepared from overnight cultures of (A) B. mallei SR1A (pvirAG) and BM0742 (pvirAG) grown in LB4G, or (B) B. mallei SR1A (pBHR2) and SR1A (pvirAG) grown in LB4G or M9G, and then assayed for Hcp1 production by Western immunoblotting using anti-BmHcp1 polyclonal rat serum. The protein band corresponding to Hcp1 is indicated by the black arrowhead.