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. 2013 Oct 11;8(10):e76767. doi: 10.1371/journal.pone.0076767

Figure 4. Quantitation of B. mallei hcp1 and virG transcript during growth in minimal media.

Figure 4

Transcript levels of hcp1, virG and dnaK were determined by qRT-PCR using gene specific primers and dual-labeled Taqman probes. RNA was harvested from B. mallei SR1A grown for 8 h in LB4G, M9G, M9CG or M9TG. Relative mRNA levels were calculated using the ΔΔCT method and represent fold changes in comparison to growth in LB4G. All values have been normalized to the internal control, rpoA. Results represent the means and standard deviations of three independent experiments performed in triplicate.