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. 2013 Oct 11;8(10):e76767. doi: 10.1371/journal.pone.0076767

Figure 5. B. mallei T6SS-1 expression in M9CG media is VirG-dependent.

Figure 5

(A) Whole cell lysates were prepared from overnight cultures of B. mallei SR1A or BM0746 (ΔvirG) grown in M9CG and then assayed for Hcp1 production by Western immunoblotting using anti-BmHcp1 polyclonal rat serum. The protein band corresponding to Hcp1 is indicated by the black arrowhead. (B) Transcript levels of bimA, tssA, hcp1, virG, tssM and dnaK were determined by qRT-PCR using gene specific primers and dual-labeled Taqman probes. RNA was harvested from B. mallei strains grown for 8 h in M9CG. Relative mRNA levels were calculated using the ΔΔCT method and represent fold changes in comparison to SR1A. All values have been normalized to the internal control, rpoA. Results represent the means and standard deviations of three independent experiments performed in triplicate. ND, not detected.