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. 2013 Oct 11;8(10):e76767. doi: 10.1371/journal.pone.0076767

Figure 8. Hcp1 is not produced by B. thailandensis during growth in minimal media.

Figure 8

Whole cell lysates were prepared from overnight cultures of B. thailandensis E264 grown in LB4G, M9G, M9CG or M9TG, and then assayed for Hcp1 production by Western immunoblotting using anti-BtHcp1 polyclonal rabbit serum. A whole cell lysate prepared from B. thailandensis DW503 (pBtvirAG) grown overnight in LB was used as a positive control (+ve) for Hcp1 expression. The protein band corresponding to Hcp1 is indicated by the black arrowhead.