Table 1. Strains, plasmids and PCR primers used in this study.
| Strain, plasmid or primer | Relevant characteristics | Reference |
| E. coli | ||
| TOP10 | General cloning strain: ApS, KmS, ZeoS | Invitrogen |
| S17-1 | Mobilizing strain; transfer genes of RP4 integrated on chromosome: PmS, KmS, ZeoS | [70] |
| B. mallei | ||
| ATCC 23344 | Type strain; human isolate from China: PmR, KmS, ZeoS | [22] |
| NCTC 3708 | Mule isolate from India | [71] |
| NCTC 3709 | Horse isolate from India | [71] |
| SR1A | ATCC 23344 derivative; sucrose-resistant: PmR, KmS, ZeoS | [17] |
| BM0742 | SR1A derivative; Δhcp1: PmR, KmS | This study |
| BM0746 | SR1A derivative; ΔvirG: PmR, KmS, ZeoS | This study |
| B. pseudomallei | ||
| K96243 | Human isolate from Thailand | [72] |
| 1026b | Human isolate from Thailand | [73] |
| B. thailandensis | ||
| E264 | Type strain (ATCC 700388); environmental isolate from Thailand | [74] |
| DW503 | E264 derivative; Δ(amrR-oprA): PmR, KmS | [75] |
| Plasmids | ||
| pEX18Zeo | Gene replacement vector; sacB, oriT: ZeoR | [17] |
| pEXZΔvirG | pEX18Zeo containing virG with an internal 210 bp deletion: ZeoR | This study |
| pMo130-ΔBPSS1498 | pMo130 derivative containing hcp1 with an internal 126 bp deletion: KmR | [50] |
| pBHR2 | Broad host range cloning vector; pBBR1 oriR, oriT: KmR | [23] |
| pvirAG | pBHR2 containing a wild type copy of B. mallei virAG (aka pBHR2-virAG): KmR | [23] |
| pBtvirAG | pBHR2 containing a wild type copy of B. thailandensis virAG: KmR | This study |
| pBAD/HisA | Arabinose inducible expression vector; pBR322 ori: ApR | Invitrogen |
| pBmhcp1-His | BmHcp1-6xHis expression construct; pBAD/HisA derivative: ApR | This study |
| pBthcp1-His | BtHcp1-6xHis expression construct; pBAD/HisA derivative: ApR | This study |
| PCR Primers a | ||
| BPSS1498-1 | 5′-GCTAGCATCCGCCAGTACGTCGTCGAC-3′ | [50] |
| BPSS1498-4 | 5′- GCTAGCTCAGGAAATCGTTCGGATATC-3′ | [50] |
| virG-RH | 5′-CATGAAGCTTAATGACGTTCGACGAGATCG-3′ | This study |
| virG-FKp | 5′-CATGGGTACCTCTTCGAGCCGTTCTATTCG-3′ | This study |
| BtvirG-FKp | 5′-CATGGGTACCAGCCGCTTCAGGCTCACGTTGC-3′ | This study |
| BtvirAG2-RXb | 5′-CATGTCTAGATCGAAATCCTCGGGAAGAAGCGCAC-3′ | This study |
| Bmhcp1-HisF1 | 5′-CCCAACGGTCTCACATGCTGGCCGGAATATATCTCAAGG-3′ | This study |
| Bmhcp1-HisR1 | 5′-CCCAACGGTCTCAAGCTTCAATGATGATGATGATGATGCGCCGCCGCGCCATTCGTCCAGTTTGCGGC-3′ | This study |
| Bthcp1-HisF1 | 5′-CCCAACGGTCTCACATGCCGATGCCGTGCTATCTCACGC-3′ | This study |
| Bthcp1-HisR1 | 5′-CCCAACGGTCTCAAGCTTCAATGATGATGATGATGATGCGCCGCCGCCACCTTCGGCGCGCGCCATGA-3′ | This study |
a
Restriction sites in the linker regions are italicized.