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. 2013 Oct 11;8(10):e77668. doi: 10.1371/journal.pone.0077668

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© 2013 Poon et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, C) TEF protein level was measured via western blot in the PVN of high-fat diet (HFD) compared to chow P15 offspring, and a statistically significant increase in total TEF was found. (B, C) Further immunoprecipitation (IP) of total TEF protein from the PVN revealed a statistically significant increase in levels of phosphorylated TEF and ubiquitin-tagged TEF. (D, E) YAP protein level was measured via western blot in the PVN of high-fat diet (HFD) compared to chow P15 offspring, and a statistically significant decrease in total YAP and phosphorylated YAP protein was found. Results are expressed as means ± SEM (n = 4) *p < 0.05. The β-actin bands were used to ensure that all samples had the same concentration of protein; they were not used to normalize TEF and YAP bands. The change in TEF and YAP band densities from the HFD group was determined relative to the chow control group. p-TEF = phosphorylated TEF; ub-TEF = ubiquitin-tagged TEF.