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. 2013 Oct 11;8(10):e77668. doi: 10.1371/journal.pone.0077668

Figure 3. Protein levels in E19 embryos.

Figure 3

(A, C) TEF protein level was measured via western blot in the PVN of high-fat diet (HFD) compared to chow E19 embryos, and a statistically significant increase was found for total TEF protein levels in whole hypothalamus. (B, C) Further immunoprecipitation (IP) of total TEF protein from whole hypothalamus revealed a statistically significant increase in levels of phosphorylated TEF and ubiquitin-tagged TEF. (D, E) YAP protein level was measured via western blot in whole hypothalamus of high-fat diet (HFD) compared to chow E19 embryos, and a statistically significant decrease in total YAP and phosphorylated YAP protein was found. Results are expressed as means ± SEM (n = 4). *p < 0.05. The β-actin bands were used to ensure that all samples had the same concentration of protein; they were not used to normalize TEF and YAP bands. The change in TEF and YAP band densities from the HFD group was determined relative to the chow control group.