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. 2013 Oct 11;8(10):e77668. doi: 10.1371/journal.pone.0077668

Figure 6. Co-localization of TEF or YAP with ENK.

Figure 6

Immunocharacterization of dissociated hypothalamic neurons from E19 embryos co-labeled with (A) TEF and ENK and (B) YAP and ENK. (C) In HFD embryos, there was a significant decrease in the density of co-labeled TEF and ENK neurons but no change in the density of co-labeled YAP and ENK neurons. (D) Knockdown of either TEF or YAP with siRNA results in a significant increase in ENK expression. Representative images of neurons are from HFD embryos. Arrows point to the same cell from images left to right (DIC, fluorescence and merged images). Results are expressed as means ± SEM (n = 4). *p < 0.05, compared with chow. TF = transcription factor; KD = knockdown; scale bar is 25 µM.