Figure 7. Targeted deletion of fgl2 leads to enhanced anti-viral T cell immunity upon LCMV WE re-challenge.

A-C) Virus-specific CD8⁺ T cell responses were analyzed in the spleen of fgl2 ^−/− or fgl2^+/+ mice, which had been infected with LCMV WE 45 days previously and were re-challenged with 2×10⁶ PFU of LCMV WE. Mononuclear cells that were isolated from the spleens of infected mice on day 2 (A) and 5 pi (B) were stimulated in vitro with class I peptides GP_33–41 or NP_396–404 to assess for anti-viral CD8⁺IFNγ⁺ T cell responses. Flow plots are representative of at least 3 mice per group. C) Graph shows the mean±SEM of at least 3 mice per group and is representative of 2 independent experiments. Statistical significance was assessed using a one-way ANOVA, * P<0.05.