Table 1. TFIID and Mediator components copurifying with ICP4.
| wtICP4 3hpi |
wtICP4 6hpi |
n208 6hpi |
wtICP4 12hpi |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M.W.a | SpCb | b/a | %C | SpC | b/a | %C | SpC | b/a | %C | SpC | b/a | %C | ||
| HSV | ICP4 | 133 | 1412 | 10.6 | 63 | 1550 | 11.6 | 66 | NA | NA | NA | 1923 | 14.5 | 66 |
| n208 | 78 | NA | NA | NA | NA | NA | NA | 2627 | 33.7 | 71 | NA | NA | NA | |
| TFIID | TAF1 | 209 | 135 | 0.65 | 38 | 100 | 0.48 | 28 | 25 | 0.12 | 14 | 51 | 0.24 | 21 |
| TAF2 | 137 | 91 | 0.66 | 45 | 66 | 0.48 | 36 | 91 | 0.66 | 45 | 40 | 0.29 | 26 | |
| TAF3 | 87 | 25 | 0.29 | 25 | 8 | 0.09 | 8.8 | 6 | 0.07 | 9.7 | 15 | 0.17 | 17 | |
| TAF4 | 68 | 68 | 1.00 | 58 | 42 | 0.62 | 50 | 37 | 0.54 | 56 | 33 | 0.49 | 49 | |
| TAF4B | 69 | 2 | 0.03 | 2.9 | 2 | 0.03 | 3.6 | 0 | 0.00 | 0 | 0 | 0.00 | 0 | |
| TAF5 | 87 | 131 | 1.51 | 69 | 66 | 0.76 | 55 | 24 | 0.28 | 21 | 76 | 0.87 | 55 | |
| TAF6 | 72 | 95 | 1.32 | 65 | 76 | 1.06 | 59 | 10 | 0.14 | 14 | 51 | 0.71 | 58 | |
| TAF7 | 40 | 15 | 0.38 | 32 | 7 | 0.18 | 21 | 3 | 0.08 | 11 | 6 | 0.15 | 17 | |
| TAF8 | 33 | 23 | 0.70 | 54 | 9 | 0.27 | 22 | 11 | 0.33 | 39 | 7 | 0.21 | 39 | |
| TAF9 | 28 | 7 | 0.25 | 65 | 2 | 0.07 | 31 | 6 | 0.21 | 64 | 0 | 0.00 | 0 | |
| TAF9B | 26 | 47 | 1.81 | 78 | 24 | 0.92 | 52 | 37 | 1.42 | 74 | 17 | 0.65 | 47 | |
| TAF10 | 22 | 9 | 0.41 | 22 | 8 | 0.36 | 25 | 7 | 0.32 | 22 | 6 | 0.27 | 22 | |
| TAF11 | 23 | 11 | 0.48 | 33 | 4 | 0.17 | 5.2 | 2 | 0.09 | 9.5 | 0 | 0.00 | 0 | |
| TAF12 | 18 | 24 | 1.33 | 32 | 14 | 0.78 | 27 | 14 | 0.78 | 27 | 19 | 1.06 | 30 | |
| TBP | 36 | 24 | 0.67 | 22 | 16 | 0.44 | 28 | 4 | 0.11 | 3.7 | 10 | 0.28 | 12 | |
| Mediator (head) | MED6 | 28 | 0 | 0.00 | 0 | 9 | 0.32 | 22 | 10 | 0.36 | 38 | 3 | 0.11 | 18 |
| MED17 | 72 | 9 | 0.13 | 15 | 16 | 0.22 | 29 | 0 | 0.00 | 0 | 18 | 0.25 | 23 | |
| MED18 | 23 | 0 | 0.00 | 0 | 7 | 0.30 | 25 | 7 | 0.30 | 14 | 10 | 0.43 | 25 | |
| MED20 | 23 | 7 | 0.30 | 19 | 14 | 0.61 | 28 | 18 | 0.78 | 30 | 14 | 0.61 | 39 | |
| Mediator (middle) | MED1 | 161 | 0 | 0.00 | 0 | 23 | 0.14 | 17 | 9 | 0.06 | 6.3 | 8 | 0.05 | 3.2 |
| MED4 | 30 | 0 | 0.00 | 0 | 8 | 0.27 | 30 | 9 | 0.30 | 25 | 4 | 0.13 | 16 | |
| MED7 | 27 | 0 | 0.00 | 0 | 8 | 0.30 | 25 | 4 | 0.15 | 22 | 0 | 0.00 | 0 | |
| MED31 | 15 | 0 | 0.00 | 0 | 6 | 0.40 | 32 | 2 | 0.13 | 20 | 7 | 0.47 | 34 | |
| Mediator (tail) | MED14 | 161 | 2 | 0.01 | 2.1 | 30 | 0.19 | 18 | 40 | 0.25 | 30 | 25 | 0.16 | 12 |
| MED15 | 76 | 0 | 0.00 | 0 | 8 | 0.11 | 10 | 0 | 0.00 | 0 | 5 | 0.07 | 7.7 | |
| MED16 | 96 | 3 | 0.03 | 3.5 | 22 | 0.23 | 19 | 2 | 0.02 | 4.1 | 18 | 0.19 | 19 | |
| MED23 | 156 | 2 | 0.01 | 1.4 | 41 | 0.26 | 26 | 33 | 0.21 | 20 | 33 | 0.21 | 21 | |
| MED25 | 83 | 0 | 0.00 | 0 | 10 | 0.12 | 11 | 0 | 0.00 | 0 | 8 | 0.10 | 7.4 | |
| MED27 | 31 | 0 | 0.00 | 0 | 8 | 0.26 | 29 | 7 | 0.23 | 29 | 5 | 0.16 | 23 | |
| Mediator (kinase) | MED12 | 244 | 11 | 0.05 | 4.7 | 58 | 0.24 | 20 | 14 | 0.06 | 6.9 | 31 | 0.13 | 15 |
| MED13 | 240 | 0 | 0.00 | 0 | 23 | 0.10 | 9.7 | 3 | 0.01 | 1.2 | 7 | 0.03 | 3.9 | |
| M13L | 242 | 0 | 0.00 | 0 | 5 | 0.02 | 2.3 | 0 | 0.00 | 0 | 5 | 0.02 | 1.7 | |
| CYC C | 35 | 0 | 0.00 | 0 | 9 | 0.26 | 27 | 5 | 0.14 | 18 | 5 | 0.14 | 12 | |
| CDK8 | 53 | 0 | 0.00 | 0 | 5 | 0.09 | 6.3 | 0 | 0.00 | 0 | 2 | 0.04 | 4.5 | |
a M.W. Molecular weight predicted from the primary amino acid sequence.
b SpC Spectral counts detected.
%C Percent coverage of the primary sequence with detected peptides.