Figure 2. The Spata4 promoter contains a functional RFX1 binding site.

(A) Nucleotide sequences of the 5′-flanking region of the Mm/hg/rn Spata4 genes. The 5′-flanking sequences are numbered with respect to the transcription start site, Mulan combined with multiTF (http://multitf.dcode.org/) scanning revealed several conserved potential binding sites of different transcription factors. (B) Effect of deletion mutants on the relative luciferase activity of the Spata4 promoter region. TM4 cells were co-transfected with the −1425/+128 Spata4 promoter construct and each deletion mutant. The results are the means ± S.E.M. of three independent experiments performed in triplicate. ***P<0.001.