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. 2013 Oct 11;8(10):e75933. doi: 10.1371/journal.pone.0075933

Figure 3. RFX1 binds to the Spata4 promoter through its DNA binding domain.

Figure 3

(A) Alignment of the conserved 14-bp sequences of human, rat and mouse Spata4 promoter with other RFX1 binding sequences shows high similarity to the RFX1 consensus binding sites. (B) Chromatin immunoprecipitation assay confirm that RFX1 could bind the conserved 14-bp sequence in mouse Sertoli cells. (C) DNA binding domain is essential for the binding of RFX1 to the conserved sequence of Spata4 promoter. Vector, pcDNA3.1 plasmid; WT-RFX1, pcDNA3.1-RFX1 plasmid; ΔDBD-RFX1, pcDNA3.1-RFX1 plasmid with deletion of the sequences encoding the DNA binding domain. **P<0.01. ***P<0.001.