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. 2013 Oct 11;8(10):e75933. doi: 10.1371/journal.pone.0075933

Figure 5. Knockdown of RFX1 could up-regulate endogenous Spata4 expression.

Figure 5

Treatment of mouse RFX1-RNAi-I, -II and -III for 48 h could efficiently knock down the endogenous RFX1 expression (A, B). (C) Spata4 mRNA expression level was elevated upon RFX1 knockdown. Control, cells treated with nonspecific siRNA. (D) RFX1 mRNA expression level was decreased by RFX1-RNAi-III treatment. (E) Spata4 mRNA expression level was significantly elevated by RFX1-RNAi-III treatment in a time-dependent manner. *P<0.05. ***P<0.001.